To investigate the effect and mechanisms of Huayu Xiaozheng Granules (HXG) on tubal epithelial cells of early tubal ectopic pregnancy. The drug-containing serum of HXG was prepared. CCK-8 assay was used to select the optium concentration. ov-Talin1 cell line was constructed by plasmid transfection. Cell adhesion capability was estimated by cell adhesion assay. mRNA and protein expression of cell adhesion related proteins E-cadherin, β-catenin, p120-catenin (p120ctn), ICAM-1, cytoskeletal proteins β-tubulin, Paxillin, Krt and signaling pathway proteins Rap1GAP, Talin1, Integrinβ3 were detected by QPCR and Western blot, respectively. The expressions of cilia-related proteins DNAH5, RSPH9, FOXJ1 and F-actin were detected by immunofluorescence staining. Results show that the drug-containing serum of HXG inhibits cell adhesion ability of ov-Talin1 tubal epithelial cells, reduces the expression of the adhesion-related proteins, decreases protein expression levels of cytoskeleton protein and cilium function related protein. The Rap1/Talin1/integrinβ3 signaling pathway are downregulated.