To promote occupational health and prevent musculoskeletal disorders among occupational drivers, the current status of functional movement ability of professional drivers was evaluated through functional movement screen (FMS), and the effect of exercise intervention was further explored. A stratified convenience sampling method was utilized to select 145 occupational drivers as participants, with their functional movement abilities rigorously evaluated using the FMS. Subsequently, 20 female drivers underwent an exercise intervention, with their exercise load monitored throughout, followed by a post-intervention FMS assessment. SPSS 29.0 software was used to analyze the FMS results, gender differences, and the effects of the exercise intervention. The results show that the FMS total scores of occupational drivers are generally below 14 points. Among the individual tests, the active straight leg raise scores highest, while the in-line lunge scores lowest. Gender differences are found in the scores for deep squat, hurdle step, active straight leg raise, and trunk stability push-up movements (P < 0.05). Post-intervention, the basic functional movements, lower limb flexibility, and trunk stability patterns of female drivers show significant improvement, with all differences being statistically significant (P < 0.05). The exercise intervention load is generally classified as moderate-intensity aerobic exercise. It is concluded that the functional movement capacity of occupational drivers is generally inadequate, and moderate-intensity exercise intervention is an effective means to improve the functional movement capacity of female drivers and prevent musculoskeletal disorders.