In order to meet the large number of future unmanned aerial vehicle (UAV) operation requirements, the safe takeoff interval for UAVs was formulated on the basis of conforming to the safety target level and aiming at the highest efficiency. According to the operating speed error characteristics of UAVs, taking into account the operating characteristics of the climb phase and cruise phase, the takeoff safety problems in three scenarios of same route operation, cross route operation and route network operation were analyzed, a collision risk assessment model was established respectively, and a calibration method for the takeoff interval was proposed in combination with Monte Carlo simulation. Finally, taking the actual operation of logistics UAVs as an example, the 10^-7 maximum collision probability was taken as the target safety level for verification, and the minimum safe takeoff interval in the three operation scenarios was analyzed and determined. The results show that the safe takeoff interval of the same route T is 122 s, the safe takeoff interval T of the cross route is related to the difference D between the distance of two takeoff points from the intersection point and satisfies T = (D ±1 199.97)/14(T≥0), and the safe takeoff intervals between the four takeoff points of the airway network system are 158, 86, 0, and 0 s, respectively. The method can provide a reference for the UAV operation enterprises to carry out takeoff interval management.