The Yingxiongling shale oil reservoirs in the Qaidam Basin are notably characterized by the development of the laminated shale and thin-layered shale. In order to identify the superior sweet spots, an experimental study on the physical and mechanical properties of laminated and thin-layered shales was conducted, and the sweet spots of the Yingxiongling shale oil reservoirs were systematically evaluated in conjunction with the analysis of oilfield production data. The results show that laminated shale exhibits higher total organic carbon (TOC) content, stronger hydrocarbon generation capacity, and higher initial oil saturation compared to thin-layered shale. Although laminated shale has lower porosity, it exhibits stronger anisotropy, higher stress sensitivity coefficient, and more developed initial natural microcracks. Additionally, the laminated shale demonstrates high horizontal permeability, strong fluid absorption capacity, and effective imbibition oil displacement ability. Its lower compressive strength facilitates the formation of complex fracture networks. The experimental research results are consistent with the in site fluid production analysis. The fracture morphology generated by the laminated shale is more complex with strong oil displacement ability and high oil displacement efficiency, indicating that the laminated shales produce fluids first; therefore, it is concluded that the laminated shales are the preferred sweet spots. The findings of this study have important theoretical significance for the exploration and development of shale oil in Yingxiongling.