Dafuling(DFL) deposit is a part of the Mingyuefeng ore field in eastern Hunan Province, China, is a typical perigranitic uranium deposit discovered recently. The characteristics of the ore minerals and the features of rare earth elements in this deposit have not been previously documented. In order to further elucidate the characteristics of ore minerals and rare earth elements (REE), as well as explore their indicative significance for uranium metallogenesis. Herein, uraninite, the primary ore mineral in the deposit, was investigated via scanning electron microscopy and electron probe microanalysis. Additionally, laser ablation-inductively coupled plasma-mass spectrometry(LA-ICP-MS) was used for the first time to determine the in situ the REE characteristics of uraninite. Uraninite exhibits a distinct fractionation between light rare earth elements(LREE) and heavy rare earth elements(HREE), while displaying a negative Eu anomaly. The (La/Yb)_N ratio exceeds 1, indicating a significant enrichment of LREE. Both major elements and REE in uraninite suggest its formation within a hydrothermal environment at temperatures ranging from moderate to low, below 350 ℃. Consequently, DFL deposit can be classified as a typical hydrothermal vein-type uranium deposit. The REE serve as indicators of the transition of ore-bearing hydrothermal fluids from high salinity to low salinity, and the hydrothermal environment gradually shifts from a reducing state to a weakly oxidizing state. These observations suggest that the ore-bearing hydrothermal fluid responsible for metallogenesis originating from the deep crust or lithospheric mantle, ascended along regional deep faults, and subsequently underwent a series of physical and chemical transformations, eventually accumulated mineralization in suitable locations within DFL deposit.