Microbial induced calcium carbonate precipitation (MICP) technology is an emerging green reinforcement technology for geotechnical engineering, which has a good application prospect in the reinforcement of loess slopes. The reinforcement of loess by MICP is affected by a variety of factors, in addition to the external environment, material properties and reinforcement methods and other factors, the calcium source, the concentration of binder, the age of the maintenance and the maintenance methods also play a decisive role in the microbial reinforcement of loess. The loess in Longxi area was taken as the research object, bacillus subtilisinduced calcium carbonate precipitation technology was adopted to consolidate loess, and a comparative experimental study on the shear strength of MICP-consolidated loess under the conditions of different calcium sources, binder concentration, age of maintenance and maintenance methods was carried out. The results show that the MICP technology is more effective in consolidating loess specimens when the calcium source is calcium chloride, the binder concentration is 1.0 mol/L and the specimens are cured for 7 d. The cohesion and internal friction are increased by 4.95 and 1.34 times, respectively, compared with the vegetal loess soil. The research results have certain reference value for the roadbed reinforcement and slope management in the Loess Plateau area.