Few systematic studies on the effect of content of soluble oils and dissolved organic matters on coagulation treatment of high suspended mine drainage. Therefore, the typical high suspended mine water was selected to analyze the effect of content of emulsified oil, concentrated liquid, HA (humic acid), FA (fulvic acid) and LA (L-tryptophan) on the efficiency of high suspended matter mine water treated by loading coagulation treatment with sand. The results show that emulsified oil, concentrated liquid, and excess FA were harmful for the efficiency and rate of loading coagulation treatment with sand. Whereas, the effect of HA, LA and lower FA content effect can be ignored. The results can effectively improve the operation technology of the loading coagulation with sand, realize the protection and rationalization of coal mine water resources.