ARGs(antibiotic resistance genes) as emerging environmental pollutants threaten human health, and studies show that secondary effluent from sewage treatment plants is an important source of ARGs in the environment. Ozone pre-oxidation combined with PAC(powdered activated carbon) and UF(ultrafiltration) was used to treat the secondary effluent from the wastewater treatment plant, and the factors and mechanisms of O₃ dosage, pH, and temperature on the removal of different forms of ARGs in the secondary effluent were investigated. The results show that ARGs could be more effectively removed by PAC adsorption and UF after oxidative inactivation by O₃; under the condition of optimal O₃ dosage (2.0 mg/L), the combined O₃-PAC-UF process could remove different types of ARGs(tetA, tetC, tetG, sulI, sulII) and other pollutants (intⅠ1, 16S rRNA) from the secondary effluent. The removal of different types of ARGs (tetA, tetC, tetG, sulI, sulII) and other pollutants (intⅠ1, 16S rRNA) by the combined process is 10^2.67 ~ 10^3.92 copies/mL, and the removal of cellular and free ARGs is significantly enhanced; the elevated pH and lower temperature facilitate the removal of ARGs; the direct oxidation of O₃ molecules dominate the removal of ARGs in the secondary effluent. In conclusion, ozone combined with PAC and UF can effectively remove antibiotic resistance genes from secondary effluent.