In recent years, due to the rapid increase in the number of motor vehicles in the market, tire wear particles (TWP) have gradually come into people's view. However, the domestic research on TWP has just started, and it is still unclear whether the typical sponge facility, biological retention zone, can effectively trap TWP. Yuelai Ecological City, Yubei District of Chongqing was selected as the research area, and soil samples of biological holdup zone were collected, TWP was extracted, and TWP in biological holdup zone was analyzed by scanning electron microscope (SEM), energy dispersive spectrometer (EDS), infrared spectroscopy, thermogravimetry, soil heavy metal analyzer and particle size analyzer. The results show that TWP is irregular block, ball and long strip. Si is a relatively high content element in TWP, while Zn content is relatively low overall. The weight of the TWP mixture extracted from the surface soil is higher than that from the middle soil and the bottom soil, and the change of thermal weight is relatively large. There are obvious weight changes around 100, 250 ~ 400, 400 ~ 550℃. The size distribution of TWP mixture is 13.11 ~116.1 μm, the average particle size is 68.62 μm.