The integration of a large number of new energy sources into the power grid has put higher demands on the operation of hydropower. To improve the regulation characteristics of hydropower, a parameter tuning method was proposed for proportional integral differential(PID) control of hydraulic turbine governor, considering the comprehensive regulation characteristics. Firstly, the hydraulic turbine regulation system model was established based on the method of characteristic and the hydraulic turbine characteristic curve. Secondly, using the non-dominated sorting genetic algorithm Ⅲ(NSGA-Ⅲ), Pareto solution sets were obtained by targeting the integrated time and absolute error (ITAE) and the overshoot of the rotational speed. The proportional, integral, and differential adjustment coefficients were used as decision variables. Finally, based on the technique for order preference by similarity to ideal solution(TOPSIS), the PID parameters with the best comprehensive regulation characteristics were finally obtained by taking the power inversion, power overshoot, power stabilization time, unit water head pole, rotational speed ITAE and rotational speed overshoot as the indexes for the load increase/decrease condition. The results show that the PID parameter tuning method can effectively improve the comprehensive regulation characteristics of the hydraulic turbine and provide a theoretical basis for the selection of PID parameters in the actual project.