To investigate how the information influence travelers' choice of non-motorized travel behavior and its degree of influence, the information intervention mechanism of non-motorized travel behavior was analyzed based on the theory of protective motivation. Through questionnaires and information intervention experiments on non-motorized travel behavior, the changes in travelers' travel behavior before and after the information intervention were studied. The impact mechanism of information intervention on non-motorized travel behavior was analyzed. The results show these as follows. Health information, environmental information, and policy guidance information all contribute to non-motorized travel, with policy guidance interventions having the best effect. Self-efficacy, severity, reflective efficacy, and cost have a significant impact on the level of willingness to engage in non-motorized travel, while demographic variables have no significant effect on the effectiveness of information intervention experiments. Information interventions can positively correlate with travellers' non-motorized travel and can be effective in inducing non-motorized travel.