心脏骤停-心肺复苏后全脑缺血再灌注损伤是急危重症常见的病理损伤，其致死率和致残率较高。目前，传统窒息法常用于构建心脏骤停-心肺复苏后脑损伤模型，但其复苏成功率和术后存活率较低。为此，通过改良传统窒息法构建心脏骤停-心肺复苏后全脑缺血再灌注损伤模型，并与传统窒息法进行比较，评估改良窒息法的优势。将SD（Sprague Dawley）大鼠随机分为传统组与改良组，分别采用传统窒息法和改良窒息法构建模型。观察大鼠复苏成功率和24 h生存率，采用神经功能损害评分评估神经功能，苏木精-伊红染色观察脑组织病理改变，透射电镜观察神经元超微结构，Western Blot检测炎症、氧化应激、凋亡指标。结果表明：与传统窒息法相比，改良窒息法构建大鼠心脏骤停-心肺复苏后脑缺血再灌注损伤模型具有更高的复苏成功率和24 h存活率，且脑损伤、炎症、氧化应激、凋亡等情况与传统窒息法比较无明显差异，能够满足全脑缺血再灌注损伤研究的需要。

Cardiac arrest and global cerebral ischemia-reperfusion injury after cardiopulmonary resuscitation (CPR) are common pathological conditions in critically ill patients, with high mortality and disability rates. Currently, the conventional asphyxia method is commonly used to establish a brain injury model after CPR. However, it has a low resuscitation success rate and postoperative survival rate. Therefore, a modified asphyxia model of cardiac arrest and global cerebral ischemia-reperfusion injury after CPR was established and compared it with the conventional asphyxia method to evaluate its advantages. Sprague Dawley(SD) rats were randomly divided into a conventional group and a modified group. The conventional asphyxia method and the modified asphyxia method were used to establish the models, respectively. The resuscitation success rate and 24-hour survival rate were observed. Neurological deficits were assessed using neurological deficit scores. Hematoxylin-eosin staining was used to observe pathological changes in brain tissue. Transmission electron microscopy was used to examine neuronal ultrastructure. Western Blot analysis was performed to detect inflammatory, oxidative stress, and apoptotic markers. The results show that compared with the conventional asphyxia method, the modified asphyxia method has a higher resuscitation success rate and 24-hour survival rate. No significant differences are observed in brain injury, inflammation, oxidative stress, and apoptosis between the two methods. The modified asphyxia method meets the requirements for studying global cerebral ischemia-reperfusion injury.