抗生素抗性基因（antibiotic resistance genes, ARGs）作为新兴环境污染物已对人类健康造成威胁，有研究表明，污水处理厂的二级出水是环境中ARGs的重要来源。采用O₃预氧化结合粉末活性炭（powdered activated carbon, PAC）与超滤（ultrafiltration, UF）组合工艺处理污水厂二级出水，考察O₃投加量与水样的pH、温度对二级出水中不同形态ARGs去除效果的影响因素和作用机理。结果表明：ARGs被O₃氧化失活后，经过PAC吸附与UF膜的过滤能得到更为有效的去除；在O₃最佳投加量（2.0 mg/L）条件下，O₃-PAC-UF组合工艺对二级出水中不同类型ARGs（tetA、tetC、tetG、sulI、sulII）和其他污染物（intⅠ1、16S rRNA）的去除量为10^2.67 ~ 10^3.92 copies/mL，且对细胞态和游离态ARGs去除效果明显增强；升高pH和较低温度均利于ARGs的去除；O₃分子的直接氧化作用居于二级出水中ARGs去除机制的主导地位。综上，臭氧联合粉末活性炭与超滤组合可以有效去除二级出水中的抗生素抗性基因。

ARGs(antibiotic resistance genes) as emerging environmental pollutants threaten human health, and studies show that secondary effluent from sewage treatment plants is an important source of ARGs in the environment. Ozone pre-oxidation combined with PAC(powdered activated carbon) and UF(ultrafiltration) was used to treat the secondary effluent from the wastewater treatment plant, and the factors and mechanisms of O₃ dosage, pH, and temperature on the removal of different forms of ARGs in the secondary effluent were investigated. The results show that ARGs could be more effectively removed by PAC adsorption and UF after oxidative inactivation by O₃; under the condition of optimal O₃ dosage (2.0 mg/L), the combined O₃-PAC-UF process could remove different types of ARGs(tetA, tetC, tetG, sulI, sulII) and other pollutants (intⅠ1, 16S rRNA) from the secondary effluent. The removal of different types of ARGs (tetA, tetC, tetG, sulI, sulII) and other pollutants (intⅠ1, 16S rRNA) by the combined process is 10^2.67 ~ 10^3.92 copies/mL, and the removal of cellular and free ARGs is significantly enhanced; the elevated pH and lower temperature facilitate the removal of ARGs; the direct oxidation of O₃ molecules dominate the removal of ARGs in the secondary effluent. In conclusion, ozone combined with PAC and UF can effectively remove antibiotic resistance genes from secondary effluent.