基于16SrDNA技术探究熟地黄对D-半乳糖致衰老小鼠的作用机制
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贵州中医药大学

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R965.1;R975.5

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:省属高校科研平台团队项目(黔教技[2022]022号);贵州省高等学校重点实验室(黔教技[2023]018号);贵州中医药大学校级研究中心(贵中医ZX合字[2024]024号)


Exploring the mechanism of Prepared rehmannia root on D-galactose-induced aging model mice based on 16SrDNA technology
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Guizhou University of Traditional Chinese Medicine

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    摘要:

    目的 探讨熟地黄对D-半乳糖诱导的衰老学习记忆障碍模型小鼠的作用及机制。方法 将实验动物随机分为5组,除空白组外,其余各组用D-半乳糖(200mg/kg)复制衰老学习记忆障碍小鼠模型,连续8周。从第5周开始,造模同时给予银杏叶片(0.01g/kg),熟地黄低、高剂量(1.95g/kg、3.90g/kg)灌胃, 空白组和模型组灌胃等容量生理盐水,持续4周后,从第9周开始用Morris水迷宫检测,病理组织学检测,免疫组化检测,生化检测,16SrDNA肠道菌群检测。结果 与空白组比较,模型组潜伏期延长,且血清SOD、 GSH-Px降低,MDA升高,其脑组织SOD、GSH-Px表达降低,而MDA表达升高;与模型组比较,熟地黄低、高剂量组潜伏期缩短(P<0.05),血清SOD、GSH-Px升高,MDA下降,其脑组织SOD、GSH-Px表达升高,MDA下降(P<0.01)。在门水平上,小鼠肠道菌群以Firmicutes和 Bacteroidetes为主(P<0.01),熟地黄低剂量组、模型组Bacteroidetes/Firmicutes(B/F)比例降低,且熟地黄低剂量组低于模型组(P<0.05),与空白组相比,模型组Proteobacteria、Epsilonbacteraeota 相对丰度显著下降(P<0.01);与模型组相比,地黄低、高剂量组Proteobacteria、 Epsilonbacteraeota的相对丰度下降( P<0.05)。在属水平上,与空白组相比,模型组Muribaculaceae-ge的相对丰度相对增加(P<0.01),模型组Helicobacter、Halomonas的相对丰度显著降低(P<0.01),与模型组相比,地黄低剂量组中Lachnospiraceae-NK4A136-group相对丰度增加(P<0.01),Muribaculaceae-ge的相对丰度降低(P<0.01)。结论 熟地黄能改善衰老小鼠的学习记忆能力,其作用机制可能与抗氧化应激及调节肠道菌群多样性有关。

    Abstract:

    Objective: To study the mechanism of Rehmannia on D-galactose induced aging model mice. Methods: The experimental animals were randomly divided into 5 groups, except the blank group, the other groups were treated with D-galactose (200mg/kg) to create aging model, starting from the 5th week, at the same time, ginkgo biloba (0.01g/kg)?low and high doses of rehmannia (1.95g/kg, 3.90g/kg) were given intragastric administration, the blank group and the model group were given intragastric administration with equal volume of normal saline, which lasted for 4 weeks, and Morris water maze was used for detection from the 9th week;the structural changes of brain tissues were observed by hematoxylin-eosinstaining;the Immunohistochemical was detected ;the oxidation indexes in liver and serum of each group were determined;the intestinal flora was detected. Results: Compared with the blank group, the incubation period of the model group was prolonged ,the serum SOD?GSH -Px were decreased , MDA was increased,and the expression of SOD and GSH-Px in the brain tissue was decreased, MDA was increased ; Compared with model group, the latency of Rehmannium group was shortened (P<0.05), SOD and GSH-Px were increased , and MDA was decreased, SOD and GSH-Px were increased in the brain tissue, MDA was decreased(P<0.01). At the Phylum level, Firmicutes and Bacteroidetes are the main Bacteroidetes in the intestinal flora of the mice (P<0.01). The proportion of Bacteroidetes/Firmicutes (B/F) in the two groups was reduced,the proportion of the rehmannia group is lower than the model group (P<0.05). Compared with the blank group,the relative abundance of Proteobacteria and Epsilonbacteraeota in the model group was significantly lower down(P<0.01), Compared with the model group, the relative abundance of Proteobacteria and Epsilonbacteraeota in the low rehmannia group was decreased (P<0.05). At the genus level,

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笪小云,曾奇,韦友辉,等. 基于16SrDNA技术探究熟地黄对D-半乳糖致衰老小鼠的作用机制[J]. 科学技术与工程, , ():

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  • 收稿日期:2024-04-11
  • 最后修改日期:2024-04-29
  • 录用日期:2024-05-22
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