The crossing of the shield tunnel through the high-speed railway will have an impact on the settlement of railway tracks, roadbeds and other structures, and seriously affect the operation of the high-speed railway. In order to study the influence of shield tunnel crossing high-speed railway on frame box culvert, roadbed and high-speed rail track settlement. Relying on Guiyang Rail Transit Line 3, a model of soil layer, frame culvert, track bed, and track was established, and the influence of shield tunneling in limestone on frame culvert, existing high-speed rail track and roadbed was analyzed. The results show that the transverse and longitudinal settlement of the frame culvert are "W" shaped, and the settlement curve is a "double butterfly" curve from the overall perspective of the settlement curve, and the peak settlement value of the frame culvert is 1.805 mm during the tunneling process. When the shield machine crosses the subgrade and the tunnel penetration, it will cause a sudden change in the subgrade settlement, and with the progress of excavation, the subgrade settlement will produce the transfer of the peak settlement value, and the peak value of the subgrade settlement is 1.753 mm. The track settlement curve of high-speed rail shows a single peak with excavation, and the width of the settlement groove is about the same as the width of the frame bridge, and the peak value of track settlement is 1.41 mm. By analyzing the influence of 8 kinds of shield tunneling pressure and 8 kinds of grouting pressure on subgrade settlement, it is found that the grouting pressure has a better control effect on settlement than the excavation pressure, and with the increase of grouting pressure, the peak value of subgrade surface settlement is decreasing, when the grouting pressure is 800kPa, the peak value of subgrade surface settlement is 1.355 mm, and the maximum deformation rate is 20.7%, and the grouting pressure and tunneling pressure of the shield machine are set to 400 kPa and 100 kPa respectively