铁腐殖酸对厌氧氨氧化过程的影响及潜在机理
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1.河北省地质环境监测院;2.河北科技大学

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X703

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国家自然科学基金(51678387);河北省人才工程资助项目(A201902019);


Effect of Fe-HA on Anaerobic Ammonia Oxidation Process and the Potential Mechanisms
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1.Hebei Geological Environmental Monitoring Institute;2.Hebei University of Science and Technology

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    摘要:

    为探究铁-腐殖酸复合物(iron-humic acid complex,Fe-HA)对厌氧氨氧化过程的影响及潜在机理,本研究对比了添加Fe-HA的RFe-HA反应器与对照RCON反应器在脱氮性能、污泥特性以及微生物菌群结构上的差异,并探讨了潜在机理。结果表明,氮负荷提升后(21d),RFe-HA的NH4+-N和NO2--N去除率分别为93.28%和88.60%,均高于RCON;运行稳定后,RFe-HA和RCON的脱氮性能基本一致;RFe-HA内颗粒污泥的比厌氧氨氧化活性(specific anammox activity,SAA)均小于RCON,污泥质量浓度则高于RCON,RFe-HA颗粒污泥的平均粒径和>0.85mm的占比也较RCON更高;相较于RCON,RFe-HA的颗粒污泥具有更高的EPS荧光强度,且胞外聚合物(extracellular polymeric substances,EPS)组分中疏水性的色氨酸类物质含量更高;第30d时,RFe-HA的Candidatus Kuenenia的丰度为26.35%,高于RCON的23.58%。可见Fe-HA的添加促进了Candidatus Kuenenia的增殖,并引起颗粒污泥EPS及色氨酸类物质分泌的增加。EPS及色氨酸类物质的增加一方面促进了颗粒污泥的形成,增大了颗粒粒径,提高了污泥质量浓度,增强了氮负荷变化时反应器的脱氮性能,另一方面阻塞了微生物与底物间的传质效率,降低了颗粒污泥的SAA。然而,增加的污泥质量浓度弥补了SAA降低导致的脱氮损失,使得稳定运行时RFe-HA和RCON的脱氮性能基本一致。

    Abstract:

    To investigate the effect and mechanism of Fe-humic acid complex (Fe-HA) on anaerobic ammonia oxidation (ANAMMOX) reactors, the differences in nitrogen removal performance, sludge characteristics and microbial community structure between RFe-HA with Fe-HA complex and RCON without Fe-HA complex were compared. The results showed that after the nitrogen load was increased (21 d), the NH4+-N and NO2--N removal efficiencies of RFe-HA were 93.28% and 88.60%, respectively, which were higher than those of RCON. After the operation was stabilized, the nitrogen removal performance of RFe-HA and RCON was basically the same. The sludge characteristics showed that the specific anaerobic ammonia oxidation activity (SAA) of granular sludge within RFe-HA was less than RCON, while the sludge concentration was higher than RCON. The average particle size and the percentage of granular sludge >0.85 mm were also higher in RFe-HA than in RCON. Compared to RCON, the granular sludge in RFe-HA had higher extracellular polymeric substances (EPS) fluorescence intensity and higher content of hydrophobic tryptophan-like substances in the EPS fraction. The community structure showed that the abundance of Candidatus Kuenenia in RFe-HA was 26.35% at 30d, which was higher than that of 23.58% in RCON. The addition of Fe-HA complex promoted the proliferation of Candidatus Kuenenia and caused an increase in the secretion of EPS and tryptophan-like substances from granular sludge. On the one hand, the increase of EPS and tryptophan-like substances promoted the formation of granular sludge, increased particle size, increased sludge concentration, and enhanced nitrogen removal performance of the reactor when the nitrogen load changes; on the other hand, they blocked the mass transfer efficiency between microorganisms and substrate and reduced the SAA of granular sludge. However, the increased sludge concentration compensated for the loss of nitrogen removal due to lower SAA, resulting in essentially the same nitrogen removal performance of RFe-HA and RCON at stable operation.

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马志远,王振毅,廉静,等. 铁腐殖酸对厌氧氨氧化过程的影响及潜在机理[J]. 科学技术与工程, , ():

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  • 收稿日期:2023-10-11
  • 最后修改日期:2024-05-20
  • 录用日期:2024-05-21
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