Sodium bicarbonate is effective in improving athletic performance and relieving athletic fatigue, but oral administration of sodium bicarbonate to athletes is associated with side effects such as alkalosis, gastrointestinal reactions, and cardiac arrhythmias, thus preventing its widespread use in competitive sports. Objective: To investigate the effects of topical transdermal administration of sodium bicarbonate on serum indices and delayed muscle soreness during high-intensity interval training in male soccer players. Methods: Sixteen high-level male soccer players were selected for the study, and after applying sodium bicarbonate topical emulsion and placebo emulsion to them daily, respectively, after 7 days of high-intensity interval training, the subjective fatigue and delayed muscle soreness of lower limbs after training were counted, and the blood biochemical indices such as blood lactate, creatine kinase, urea nitrogen and hemoglobin before and after training, the amount of training stimulus during the training period, the maximum heart rate, energy expenditure, aerobic and anaerobic training effect indexes, running distance, running speed, acceleration, high speed running distance, total load and other sports performance indexes were analyzed. Results: Before and after a high-intensity interval training, the experimental group showed significantly lower blood lactate elevation (p< 0.05), higher creatine kinase elevation and lower blood urea nitrogen elevation compared to the control group; during the 7-day training period, the overall lower extremity DOMS and RPE levels were significantly lower in the experimental group compared to the control group (p< 0.05), and the lower extremity DOMS values were significantly The overall level of lower limb DOMS and RPE in the experimental group was significantly lower than that in the control group (p< 0.05), and the lower limb DOMS values were significantly lower on day 2 and day 6 (p< 0.05). The anaerobic training effect, peak EPOC, maximum heart rate, and energy expenditure of the experimental group athletes training were all improved compared with the control group, among which maximum heart rate and anaerobic training effect were significantly improved (p< 0.05) Conclusion: The topical administration of sodium bicarbonate can significantly reduce the delayed muscle soreness brought about by high-intensity interval training of soccer players, and significantly reduce the blood lactic acid produced by high-intensity training It is conducive to the enhancement of anaerobic training effect after high-intensity training, resulting in the enhancement of athletes"" heart rate reserve capacity and rapid recovery capacity, thus promoting the improvement of special athletic ability.