School of Environmental and Safety Engineering, North University of China
Key Research and Development Program of Shanxi Province(201903D321083);Central Guide Local Science and Technology Development Fund Projec(YDZX 20201400001443);Graduate Science and Technology Project of North University of China (20201767), Innovation and Entrepreneurship Project of Students of North University of China (20210381);
来自焦化废水环境的多环芳烃降解菌弗留明拜叶林克氏菌Beijerinckia fluminensis 6-1H能够高效降解菲，为揭示菲生物降解的机理，对其降解途径和酶比活力进行分析。通过UV-Vis、GC-MS以及FTIR技术以及菲降解过程中水杨酸及邻苯二甲酸浓度变化分析，初步鉴定出菌株6-1H降解菲的重要的中间代谢产物：1-羟基-2-萘甲酸、1-萘酚、水杨酸、龙胆酸、邻苯二甲酸、马来酰丙酮酸、原儿茶酸和β-胡萝卜素。通过菌株6-1H代谢菲的酶比活力测定，发现菌株通过1-羟基-2-萘甲酸羟化酶、1-萘酚羟化酶、邻苯二甲酸双加氧酶和水杨酸-5-羟化酶降解菲。依据代谢产物检测和酶活力检测分析结果，推测菌株6-1H降解菲经由菲C3,4-双加氧途径，生成1-羟基-2-萘甲酸和1-萘酚，然后通过代谢水杨酸生成龙胆酸，继续降解为马来酰丙酮酸直至完全矿化；或者1-萘酚通过过代谢邻苯二甲酸生成原儿茶酸，进一步降解生成β-胡萝卜素及H2O、CO2。本研究不仅初步探明菌株6-1H对菲的降解机理，也为菲污染的生物修复技术提供了理论依据。
Beijerinckia fluminensis 6-1H isolated from coking wastewater environment was effective in degrading phenanthrene. The degradation path and specific activity of enzyme were analysed in order to find out biodegradation mechanism. Metabolites detection by UV-Vis, GC-MS, FTIR and concentration variation of salicylic acid and phthalic acid revealed eight important metabolites, 1-hydroxy-2-naphthoate, 1-naphthol, salicylic acid, gentianic acid, phthalic acid, maleoyl pyruvic acid, pureonebio and β-carotene , generated during phenanthrene degradation by strain 6-1H. Specific activity of enzyme analysis revealed that four kinds of enzymes involved in phenanthrene degradation contained 1-hydroxy-2-naphthoicacid hydroxylase, 1-naphthol hydroxylase, phthalic acid dioxygenase and salicylic acid-5 hydroxylase during phenanthrene degradation. Based on the metabolic data and the specific activity of enzyme analysis, here we proposed a pathway involved C3,4-dioxygenation for phenanthrene degradation by strain 6-1H, generated metabolites 1-hydroxy-2-naphthoate and 1-naphthol. Then form gentianic acid through salicylic acid, and gentianic acid continues to be degraded to maleoyl pyruvic acid until complete mineralization. Or 1-naphthol continues to be degraded to phthalic acid, finally generating β-carotene, H2O and CO2 through protocatechuic acid. This study expands our understanding of the degradation mechanism of phenanthrene degradation, which can be useful for the bioremediation applications.
白红娟,卫燕红,张晴,等. 弗留明拜叶林克氏菌6-1H对多环芳烃菲的生物降解机理[J]. 科学技术与工程, , ():复制