With the continuous increase of natural gas demand in winter, the peak-shaving capacity of gas storage is put forward a severe test. As a channel connecting ground and underground, wellbore temperature changes under the influence of injection and production cycle. How to accurately predict wellbore temperature changes is very important to predict annulus pressure, ensure the safety of tubing and casing, cement ring sealing and gas storage operation safety. According to the law of mass conservation, energy conservation and momentum theorem in fluid mechanics, the pressure, temperature, density and velocity of fluid are coupled. By using the explicit fourth order Runge-Kutta numerical solution method, the flow Parameters of different depth of fluid in tubing are outputted, and the sensitivity factors affecting gas temperature in tubing are analyzed. The results show that the temperature at the bottom of the well is greatly affected by the gas injection, and the gas injection time and pressure have little effect on the bottom of the well; In the process of gas production, the gas temperature at the wellhead increases with the increase of gas production, the gas pressure in the tubing decreases gradually from the bottom to the wellhead, and the decrease trend becomes more obvious with the increase of gas production; In the early stage of shut-off, the gas temperature in the tubing changes at a large rate. With the continuous increase of shut-off time, the gas temperature in the tubing gradually recovers to the formation temperature, and the closer it is to the wellhead gas temperature change rate, the more obvious it is.