In order to explore the influence of the clocking position matching relationship between the impeller and the spatial guide vane on the performance of the well submersible pump, taking the 200QJ20 multi-stage submersible pump as the research object, the secondary impeller and the secondary spatial guide vane are rotated respectively at the clocking position of 1/2 of the angle between the two adjacent blades, and four different clocking schemes are combined. The CFD method is used to simulate the steady and unsteady flow field in each model. The results show that when the position of the time sequence is different, the difference between the maximum head and the minimum head is 0.5m, and the difference between the maximum efficiency and the minimum efficiency is 0.5%. When the secondary impeller rotates 1/2 of the impeller blade angle and the secondary space guide vane does not change, the head and efficiency are the maximum. the high pressure area close to the guide vane face is the largest, and the flow line in the impeller passage area is more smooth. When the secondary impeller rotates the blade angle of 1/2 impeller and the secondary space guide vane rotates 1/2, the lift and efficiency are the smallest, and the high pressure area close to the guide vane working face is the smallest, and there is a secondary eddy current region on the back of the guide vane. the pressure pulsation amplitude at the inlet and outlet of the impeller and the guide vane decreases obviously, while the pressure pulsation amplitude at the exit of the guide vane increases obviously.