A field experiment was conducted to investigate effects of seed dressing with Trichoderma spp. on wheat growth and rhizosphere fungal community. The wheat rhizosphere soil was collected after sowing 180 days, and the effects of four different strains of Trichoderma on fungal community in wheat rhizosphere soil were studied by high-throughput sequencing technology, the absolute content of fungi was detected by qPCR. The results showed that the abundance of fungal community in wheat rhizosphere soil was decreased by Trichoderma spp. treatment. The wheat yield was inversely proportional to the uniformity (Shannon index) and richness (OTUs number) of fungal community in rhizosphere soil, but positively proportional to the dominance (Simpson index). The white head rate, caused by wheat sharp eyespot and crown rot, was decreased compared with non-dressing treatment. The control effects of Trichoderma harzianum LTR-2 and QT21990 were more than 60%, and the yield increased by 7.42% and 6.94% (P<0.05), respectively.