In order to explore the influence of the track sensitive wavelength on the operation of modern trams, the dynamic response index of a modern tram with the independent wheels under different wavelengths excitations was analyzed to obtain the sensitive wavelength range of the tram; Then, the irregular excitation of track was generated, and the parameter changes of vehicle passing before and after the excitation were analyzed. When the tram runs in a straight line, the direction and horizontal irregularity mainly affect the lateral acceleration, and the longitudinal irregularity mainly affect the vertical acceleration. When the vehicle runs on a curve, the direction and horizontal irregularity affect the low-speed and high-speed curve passing performance of the tram respectively. The combination of the two has a comprehensive effect. The longitudinal irregularity mainly changes the running state of the trolley curve of high speed. The impact of the direction and horizontal reverse irregularity of the tram’s safety will decrease with the increase of the wavelength, and the reasonable curve can reduce the impact. By analyzing the relationship between the sensitive wavelength and the operation of tram, it has guiding significance for tram to pass through different excitation sections.