茉莉酸处理对木本油料植物小桐子早期花序芽基因表达的影响
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Effects of exogenous jasmonic acid on gene transcription in early inflorescence buds of the woody oil plant Jatropha curcas
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    摘要:

    小桐子 (Jatropha curcas) 是大戟科多年生木本油料植物,其种子含油率较高 (30-40%),是一种潜在的可再生能源植物。然而小桐子雌花较少,种子产量较低,严重限制其推广应用。茉莉酸是一种重要的植物激素,在花发育过程中起着重要作用。为了解茉莉酸在小桐子花发育过程中的作用,应用第二代高通量测序方法,对1.0 mM茉莉酸处理24小时后的小桐子早期花序芽进行转录组测序分析,观测茉莉酸对花发育及相关基因转录的影响。结果显示,外源茉莉酸处理导致早期花序芽中1259个基因上调表达,695个基因下调表达;其中,10个与成花转变相关,8个与花器官发育相关以及18个与茉莉酸合成和信号转导途径相关的拟南芥同源基因表达发生了显著变化,但是花器官表型并未有显著的改变。差异表达基因的GO注释显示,“响应茉莉酸”功能分类基因在上调表达基因群中富集,表明在小桐子中存在可以响应外源茉莉酸处理的信号途径。茉莉酸处理后,与拟南芥花器官发育相关的同源基因JcFUL、JcSRS、JcSEP1、JcAGL61、JcWOX1、JcTPR4和JcSEU下调表达,然而小桐子花器官表型没有明显改变,说明在小桐子中这些基因的变化不足以改变小桐子花器官表型。实验结果对解析茉莉酸在小桐子花发育调控过程中的作用有一定的参考价值。

    Abstract:

    Jatropha curcas is a woody perennial oil plant of the family Euphorbia, whose seeds contain high oil content (30-40%), and is considered as a potential renewable resource plant. However, few female flowers and low seed yield of J. curcas severely limit its application in agricultural production. Jasmonic acid (JA) is an important plant hormone, which plays important roles in flower development. To investigate the role of JA in flower development, the changes of transcriptome in the early inflorescence buds of Jatropha 24 h after treatment with 1.0 mM JA were detected using second-generation high-throughput sequencing technology. The results showed that 1259 genes were up-regulated expression and 695 genes were down-regulated expression in the inflorescence buds treated with JA, among which 10 orthologous genes of Arabidopsis floral transition genes, 8 orthologous genes of Arabidopsis floral organ development genes and 18 orthologous genes of Arabidopsis JA synthetic and signaling transduction pathways exhibited significantly changed expression, but the phenotype of floral organs was normal. GO annotation of differentially expressed genes (DEGs) showed that genes in the “responding to jasmonic acid” functional category are enriched in the up-regulated DEG group, indicating that the signaling pathways responding to the exogenous JA application are present in Jatropha. Moreover, after JA treatment the expression of JcFUL, JcSRS, JcSEP1, JcAGL61, JcWOX1, JcTPR4 and JcSEU genes related to floral organ development were down-regulated, but floral organ phenotype was normal, indicating that the down-regulated expression of these genes is not sufficient to alter floral organ phenotype in Jatropha. These results provide valuable information for analyzing the role of jasmonic acid in the regulation of floral development of Jatropha.

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徐传佳,陈茂盛,徐增富. 茉莉酸处理对木本油料植物小桐子早期花序芽基因表达的影响[J]. 科学技术与工程, 2021, 21(14): 5742-5751.
Xu Chuanjia, Chen Maosheng, Xu Zengfu. Effects of exogenous jasmonic acid on gene transcription in early inflorescence buds of the woody oil plant Jatropha curcas[J]. Science Technology and Engineering,2021,21(14):5742-5751.

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  • 收稿日期:2020-09-27
  • 最后修改日期:2021-05-31
  • 录用日期:2020-12-21
  • 在线发布日期: 2021-07-07
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