To compare the content of bolbostemmae glycoside A of rhizoma bolbostemmae planted under the condition of pseudo-wild with one by domestic species sold from market,by means of active component, bolbostemmae glycoside A for quality control target is used. The RP-HPLC and 65% methanol for mobile phase were utilized. Flow rate was 1.0 mL/min, detection wavelength was 214 nm. It is Considered that the content of bolbostemmae glycoside A of rhizoma bolbostemmae planted under the condition of pseudo-wild and one by domestic species sold from market were 1.776% and 1.009%. The recovery rate was 98.79%,RSD=1.77%(n=5).It is conclued that the content of bolbostemmae glycoside A of rhizoma bolbostemmae planted under the coundition of pseudo-wild was higher than one by domestic species sold from market.