To express the chimeric gene containing Hantaan virus S and M segments in prokaryotic expression system and compare the activities of the chimeric genes constructed by different splicing ways. The prokaryotic expression vector pGEX-4T-1-S0.7G2 was constructed. After inducing expressed the fused protein, Western-blot and ELISA methods were used to compared the activities of N0.7G2 with G2N0.7. The results showed that the activities of N0.7G2 was lower than that of G2N0.7. It is suggested that the splicing ways of chimeric genes may affect the activities of fused proteins expressed by the chimeric genes.