Multi-channel vibrating wire sensor frequency measurement system usually adopts the way of time-sharing asynchronous measurement, which can hardly meet the measurement needs of dynamic responses of bridges and other mega structures. In response to it, a set of multi-channel vibrating wire sensor synchronous frequency measurement system is developed on basis of a synchronous measurement method integrating simultaneous vibration and time-sharing measurement given in the chapter. Centering on Cortex-M4 processor, the system uses Rife-Quinn algorithm to figure out the frequency. Experimental test result of the system indicates that it can complete synchronous measurement of four-channel vibrating wire sensor, and its relative error of frequency measurement in noiseless condition is less than 0.007%; Input sinusoidal signal with a frequency of 1KHz and a peak ranging from 2μV to 3.4mV, the system’s relative error of frequency measurement is less than 0.005% and its standard deviation is less than 0.04Hz; When white noise interference is imposed, the system’s SNR ranges from -9dB to 9dB, and its relative error of frequency measurement is less than or equal to 0.2%.