In drilling engineering, in addition to long-term bearing the fatigue damage caused by axial pressure, the drill pipe is also under the influence of oil and gas corrosion environment. Oil and gas production is often accompanied by a large number of H2S production. In this paper, the rotating bending fatigue testing machine is used to carry out the fatigue test of S135 material which is commonly used in drilling, and the H2S corrosion test is carried out with reference to the TM0177-2005 NACE standard , The corrosion time was 48H and 96H respectively, and the corrosion test specimens were fatigue test, the S-N curves were obtained. The fatigue fracture mechanism under different corrosion time was observed by scanning electron microscope. The experiments show that the influence of the corrosive medium on the surface roughness of the specimen is the most important factor that affects the fatigue life, and the influence of the surface roughness on the fatigue life isgradually increased with the decrease of the stress.